Study of the Different Gamma – Irradiation Doses Effect on the Survival and Biosurfactant Activity of Bacteria Genus Rhodococcus

Abstract

The research on studies of the various gamma-irradiation doses effect for survival and ability of mutant strains to synthesize biosurfactants has been conducted. It was shown that gamma-irradiation with inducing dose in 2-3 kGr for bacteria of the genus Rhodococcus, more than 1.4 times increasing of biosurfactant biosynthesis and surviving of cells from 0.021 to 0.15% in this radiation dose has been observed. It was found that increasing of the gamma-irradiation dose up to 4-5 kGr decreases the surviving cells titer, but are not lethal for bacteria of the Rhodococcus genus. The sensitivity of the strains was observed at the 10 kGr irradiation dose, after inoculation of irradiated strains at doses of 10-20 kGr, an absolute inactivation of microorganisms occurred, and was confirmed by the absence of strain growth. The sensitivity of the strains was observed to an irradiation dose of 10 - 20 kGr. When seeding strains 168 hours after irradiation with these doses, a complete death of microorganisms was observed, which was confirmed by the absence of growth of strains.