Amlodipine (P-glycoprotein) inhibitor modulation effect on the Hematological, Oxidative and Inflammatory effects of Doxorubicin in Colorectal cancer induced in Mice

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Hassan Adheem Abbas, Duraid A Abass

Abstract

An experiment was performed to assess the effect of P-gp inhibitor Amlodipine (AML) when given alone or combined with Doxorubicin (DXO) therapeutically against Colorectal cancer (CRC) induced by Azoxymethane (AOX) in mice. Forty-eight adult Albino mice equal were divided into six groups consisting of C-ve given NS and five groups treated after CRC induction according to dosing regimen (C+ve (AOX 10 mg/kg and NS), T1 (AML 1.8 mg/kg), T2 (DOX 5mg/kg), T3 (AML1.8 mg/kg with DOX 2.5mg/kg/) and T4 (AML 1.8 mg/kg with DOX 5 mg/kg). Dosing continued for four weeks followed by two weeks recovery through which blood were collected to evaluate their hematological oxidative and inflammatory effects. Hematological result recorded significant reduction in the blood RBC, PLT count and HCT percentage mainly in T1 (DOX) and T4 groups in comparison with the other experimental groups that showed nearly normal level both at end of treatment and recovery periods. While Differential (WBC) count showed significance increase in C+ve group and significance decrease in (T1, T4 and T3). Plasma GSH Concentration recorded significantly less reduction in T2 and T3 groups than other treated groups compared with control one at both periods while IL-6 concentration showed in all treated group significant increase with less effect recorded in T2 and T4 compared with control one at both end of treatment and recovery periods.

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