Anti-Inflammatory Approach of Cissus Quadrangularis Linn. By In-Vitro Means

Abstract

Background: As the modern era has marked the discovery of various synthetic and semi synthetic chemical entities for aliment of diseases, so are they associated with numerous side effects. To overcome this severe side effects after chronic use and to us the synergistic combined effect of the various Phyto ingredients of the respective drug, the extracts of the crude drugs are made to use.

Objective: The objective of the study was to in vitro analyze anti-inflammatory property of C. quadrangularis leaves using by in-vitro three assays viz. Membrane stabilization test (Blood Serum). Inhibition of albumin denaturation (egg albumin). Inhibition of protein denaturation (bovine serum albumin).

Results: The extracts of C. quadrangularis is estimated The above assays simulate the exact inflammatory physiological conditions associated with arthritis and other inflammatory conditions, and hence the results shown by it can be applicable to the anti-inflammatory activity of the drug. The anti-inflammatory in-vitro assays namely Membrane stabilization test (Blood Serum), Inhibition of albumin denaturation (egg albumin) and Inhibition of protein denaturation (bovine serum albumin) were performed and showed 88%, 84% and 82% inhibition of inflammation rate respectively when compared with diclofenac as standard on invitro basis.

Conclusion: All this established a high level of guarantee that Cissus Quadrangularis an effectively control inflammation associated with various diseases or disorders and hence can serve as promising drug in the treatment of the same.