Measuring Toxicity of Aspergillus niger isolated from different sources and effect of Agaricus bisporus filtrate and sodium bicarbonate on their toxin

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Noor Jasim Mohammed, A. S. Saadon

Abstract

The present study aimed at performing of fungi isolate from (soil from streets, dried fruit). Where the fungi accompaining the local and imported dried fruits were isolated and diagnosed, and the fungal isolates that have the ability to produce toxins, specifically Ochratoxin A, were selected. The following fungal species were isolated:


(Aspergillus niger, A.parasiticus, A.flavus, Rhizopus stolanifer, Pencillium natatum) the relative frequency of each type was calculated, and it was found that there were significant differences, and the highest frequency rate belonged to the fungus A.niger. The HPLC test was conducted for a group of fungal isolates to investigate the presence of mycotoxins, and the results showed that the highest concentration of ochratoxin A, which reached (41.89 ppb), which resulted from the fungus A.niger.   where the Agaricus bisporus fungus was used as a bio-resistance agent, and when conducting an antibody test, it was proved that the fungus Agaricus bisporus has an antidote ability against the poisonous fungus A.niger.


The results are showed its ability to significantly reduce dry weight when testing the effect of Agaricus bisporus filtrate on A.niger fungus in liquid medium (PDB) by increasing the concentration of filtrate, and the effect of sodium bicarbonate was tested on both radial growth in solid media (PDA) and dry weight. For A.niger growing in a liquid medium (PDB), where the results demonstrated the high ability of sodium bicarbonate to inhibit radial growth and reduce the dry weight of the fungus, as the results proved that the higher the concentration of sodium bicarbonate and the fungus filtrate Agaricus bisporus, the greater the rate of inhibition of the poisonous fungus.

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