Physicochemical And Biological Properties of Chitosan Extracted from Fish Scales of Labeo Rohita

Abstract

The current research was aimed to extract chitin and chitosan from the by-products (scales) of L.rohita by a sequence of chemical processes involving demineralization, deproteinization and deacetylation and studied its various physicochemical and biological properties. The most effective concentration for producing the best results was determined after considering several acid and alkali treatments. In the current research the features that make chitosan useful included Solubility (2.972), Fat Binding Capacity (111%), Water Binding capacity (106 %), and Ash Value (0.371%) have indicated the sample as good source of chitosan. The antibacterial property of chitosan against different pathogens like Pseudomonas fluorescens, Aeromonas hydrophila, Staphylococcus aureus, and Escherichia coli were evaluated. However Aeromonas hydrophila showed good antibacterial activity as compared to others. Antioxidant activity DPPH and Anti-inflammatory activity confirmed the antioxidant and anti inflamative properties of chitosan.