Evaluation of Antioxidant and Antiproliferative Activities of L-Asparaginase Produced by Pseudomonas aeruginosa

Abstract

L-asparaginase is an antineoplastic enzyme that, is often used to treat acute lymphoblastic leukaemia (ALL). Pancreatic cancer, acute myeloid leukaemia, and lymphosarcoma. It had properties; such as antioxidant and anticancer. In the present study properties of purified L-asparaginase were analyzed in vitro. The effect of enzyme toxicity on human blood components was evaluated by hemolytic and in vitro cytotoxicity assays. Free radical scavenging potentials of the purified L-asparaginase against DPPH were confirmed based on its stable antioxidant effects. The in vitro antiproliferative activity of the purified L-asparaginase against breast cancer (MDA-MB-231cell line) with IC50 value 88 µgmL-1. The enzyme demonstrated increased activity, selectivity, and antiproliferative efficacy in cancerous cells and weak cytotoxicity in normal cells (WRL68). The findings of this study indicate that the purified L-asparaginase II could be utilized in medicinal applications.