In vitro, effect of sucrose concentration and type of culture medium on fungal colonies infections on Murashige and Skoog medium

Abstract

This study was conducted in the laboratories of the College of Agriculture and Marshlands, University of Thi Qar, South of Iraq. The paper presents the effect of sucrose concentration and culture medium on the colonies of fungal infections in MS medium (Murashige and Skoog, 1962) basal medium supplemented with ‎four ‎variants of sucrose (0 control, 10, 20 and 30) gm/l as carbon source were tested. Two types of MS(1. solid 7 g/l of agar was added 2. liquid) were used, without  any hormones and vitamins. For the purpose of fungal growth, the culture media were exposed to laboratory air for two hours, then the samples were incubated at a temperature of 27 °C for 7 days. Results shown there are 5 genuses (Aspergillus spp, Rhizopus spp, Fusarium spp, Mucor spp and Penicillium spp) that grow mainly on MS culture medium. Also, the results confirmed the existence of a significant correlation between fungal growth and sucrose concentrations, the comparison treatment 0 g/L (control) did not record any fungal growth, while (30 g/l) concentration was recorded the highest growth rate (liquid:31.30 mm and solid:19.60 mm).