Detection of the, hlyA, fimH PgaD gene in Escherichia coli isolated from different sources in Kirkuk city

Abstract

This study was directed for the period from November 2021 to April 2022, where (155) clinical samples were collected from diverse patients who suffer from various pathological injuries, and they included burn injuries (25)specimen, and wounds (25) specimen, and urinary tract infections, urine specimen (50) specimen, and sputum specimen (55) specimen , This study came to discover some genes of virulence elements for bacteria Escherichia coli, Bacterial isolates analyzed using traditional methods, and then their identification was confirmed by molecular detection of hlyA, fimH, and PgaD genes by polymerase chain reaction (PCR) technology, as (42) clinical isolates belonging to the type E. coli were obtained, and the results showed that (39) isolates with a rate of (92.86%) possessed PgaD genes . (29) isolates (69.05%) possessed the hlyA gene and (37) isolates (88.10%) possessed the fimH gene. The study showed that all E. coli isolates isolated from wounds and sputum contained the PgaD gene with a percentage of (100%), and the isolates of E. coli isolated from the urine (18) isolates and from burns (6) isolates contained the PgaD gene by (90%) and (85.7%), respectively. E. coli isolates isolated from the urine (15) isolates (75%) and from burns (5) isolates (71.43%) and from sputum (3) isolates (60%), and from wounds (6) isolates (60%) contained the hlyA gene, and all isolates of E. coli isolated from sputum (5) isolates (100%) contains the FimH gene, and 19 isolates (95%) isolated from the urine, and (6) isolates (85.71%(The isolates from burns and (7) isolates (70%) from wounds contain the Fim gene.